Composite

Part:BBa_K2638013

Designed by: Erika Schneider   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


Weak constitutive expression system for CopC and CopD

Weak constitutive expression of CopC (BBa_K2638001) and CopD (BBa_K2638002) with weak anderson promoters J23115 and RBSs BBa_B0034 and a double terminator (BBa_K823017).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 566
    Illegal NheI site found at 589
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 899
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 365
    Illegal AgeI site found at 419
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 307
    Illegal BsaI.rc site found at 885


[edit]
Categories
Parameters
None